1/2/2024 0 Comments Capto mmc impres![]() ![]() Capto MMC ImpRes can typically be used in the initial polishing step in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). Contaminants such as DNA, host cell proteins (HCP), leached protein A, and aggregates, are efficiently removed, making Capto MMC ImpRes an excellent choice for purification of MAbs and other biomolecules with high aggregate content. The multimodal functionality of Capto MMC ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength. Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity. 10 cm bed height of HiScreen columns allows method optimization and parameter screening. The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted. Displays a broad pH/salt operational window. Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). Application Capto SP ImpRes has a strong sulfonate cation exchanger coupled to a chemically modified, high-fLow Agarose matrix. Enables use of a platform approach to MAb process development. HiTrap ® Capto SP ImpRes is a strong cation exchanger prepacked with BioProcess Capto ImpRes media for screening and small-scale protein purifications using ion exchange chromatography (IEX). These ReadyToProcess chromatography columns are prepacked with Capto MMC ImpRes chromatography. High yields achieved through the high-resolution beads and selectivity of the ligand. Cytiva - Capto MMC ImpRes - Chromatography Resin by Cytiva. The columns are an excellent choice for method optimization and parameter screening. HiScreen Capto MMC ImpRes HiScreen Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere MMC weak cation exchange multimodal medium. The multimodal functionality gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength in combination with high resolution- and yield. The column is an excellent choice for method optimization and parameter screening. Ready to use ion exchange column, pre-packed with Capto adhere ImpRes multimodal medium. Capto MMC ImpRes multimodal chromatography resin Designed for modern, large-scale manufacturers, this resin is used for high-resolution polishing of monoclonal antibodies (mAbs) and other biomolecules. In addition, recommended screening strategies are presented where a sequential approach and Design of Experiments are used and discussed.HiScreen Capto MMC ImpResHiScreen Capto MMC ImpRes To use the full potential of Capto S, we show how to thoroughly screen the loading parameters, conductivity and pH. Capto S is a strong cation exchanger designed for capture and intermediate purification of recombinant proteins and as a second step in MAb purification. Previously launched products on this platform include Capto Q, Capto MMC and the MabSelect™ family. The medium combines high rigidity with high dynamic binding capacity (DBC) and fast mass transfer to allow faster purification, more flexible process design and maximum cost efficiency. This has recently been used in the development of a new cation exchange medium, Capto™ S, and gives a substantial increase in binding capacity compared to the corresponding media without dextran.Ĭapto S is a chromatography medium based on the novel high flow agarose platform. A technique for significantly improving the capacity and mass transfer properties of ion exchange media is modification of the porous base matrix with a surface extender such as dextran. Increasing expression levels of recombinant proteins and the need for improved productivity and overall process economy puts extra demand on the next generation of chromatography media for the bio-pharmaceutical industry. ![]()
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